Carbon blacks as EPR sensors for localized measurements of tissue oxygenation.
نویسندگان
چکیده
New electron paramagnetic resonance (EPR) oximetry probes were identified in the class of carbon black materials. These compounds exhibit very high oxygen sensitivity and favorable EPR characteristics for biological applications. At low pO(2), the linewidth is particularly sensitive to changes in oxygen tension (sensitivity of 750 mG/mmHg). The application of the probes for oximetry was demonstrated in vivo: the pO(2) was measured in muscle in which the blood flow was temporarily restricted as well as in tumor-bearing mice during a carbogen breathing challenge. The responsiveness to pO(2) was stable in muscle for at least 3 months. No toxicity was observed using these materials in cellular experiments and in histological studies performed 2, 7, and 28 days after implantation. In view of their EPR characteristics (high sensitivity) as well as the well-characterized production procedure that make them available on a large scale, these probes can be considered as very promising tools for future developments in EPR oximetry.
منابع مشابه
Assessment of tumor oxygenation by electron paramagnetic resonance: principles and applications.
This review paper attempts to provide an overview of the principles and techniques that are often termed electron paramagnetic resonance (EPR) oximetry. The paper discusses the potential of such methods and illustrates they have been successfully applied to measure oxygen tension, an essential parameter of the tumor microenvironment. To help the reader understand the motivation for carrying out...
متن کاملUsing ANN and EPR models to predict carbon monoxide concentrations in urban area of Tabriz
Background: Forecasting of air pollutants has become a popular topic of environmental research today. For this purpose, the artificial neural network (AAN) technique is widely used as a reliable method for forecasting air pollutants in urban areas. On the other hand, the evolutionary polynomial regression (EPR) model has recently been used as a forecasting tool in some environmental issues. In ...
متن کاملSize Modification of Nanographite System of Activated Carbon Fibers Studied by EPR
We report results of EPR measurements of activated carbon fibers. Experiments made for pristine activated carbon fibers and activated carbon fibers with adsorbed molecules (CCl4, C6H5NO2, and H2O) confirmed the localized character of paramagnetic centers observed in the system. Pristine activated carbon fibers are characterized by single Lorentzian line. Broader component of EPR signal appears ...
متن کاملNoninvasive measurement of anatomic structure and intraluminal oxygenation in the gastrointestinal tract of living mice with spatial and spectral EPR imaging (oximetryymagnetic resonance imagingymetabolismyactivated charcoalyfree radical)
EPR imaging has emerged as an important tool for noninvasive three-dimensional (3D) spatial mapping of free radicals in biological tissues. Spectral–spatial EPR imaging enables mapping of the spectral information at each spatial position, and, from the observed line width, the localized tissue oxygenation can be mapped. We report the development of EPR imaging instrumentation enabling 3D spatia...
متن کاملElectron Paramagnetic Resonance (EPR) studies on tumor oxygenation
The aim of this review is to show how Electron Paramagnetic Resonance (EPR) and related methods such as dynamic nuclear polarisation (DNP) can be used to detect, localize and quantify oxygen concentration in biological tissues. Several applications will be described with special emphasis on tumor oxygenation. Introduction It is well established that the partial pressure of oxygen (pO2) plays im...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Magnetic resonance in medicine
دوره 51 6 شماره
صفحات -
تاریخ انتشار 2004